Beilstein J. Nanotechnol.2021,12, 93–101, doi:10.3762/bjnano.12.8
(NTA) terminal groups for the interaction with the His-tagged PMs forming a quasi-monolayer of His-tagged PM on top of the CNM-NTA. The formation of the Ni-NTA/His-tag complex leads to the unidirectional orientation of PM on the CNM substrate. Electrophoreticsedimentation was employed to optimize the
; electrophoreticsedimentation; proton pump; purple membrane; Introduction
The so-called purple membrane (PM) has attracted large attention in the scientific literature after this light-driven proton pump was first discovered in the 1970s [1][2]. The membrane patches of about 1 µm in diameter, consisting of the
optical properties as well as their chemical [39], thermal [40], and mechanical stability [41]. PM patches were deposited by electrophoreticsedimentation from a suspension as a quasi-monolayer on a 4′-nitro-1,1′-biphenyl-4-thiol (NBPT) CNM [42][43]. We use the term quasi-monolayer to express that there
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Figure 1:
WT PM deposited on NBPT CNM using drop-casting and electrophoretic sedimentation: (a, b) initial dr...